Molecular Marker and Test Cross Information Aid Selective Advancement of F4 Generation of CB174R/Azucena: An inter Sub-specific Cross in Rice for Restorer Development

The present investigation was aimed to develop restorer lines for three-line hybrid rice using indica/tropical japonica derivatives exploit the inter sub-specific heterosis. From 75 F4 families of CB174R/Azucena, two plants were randomly selected and screened simple sequence repeat markers DRRM-RF 3 -10 Rf3 gene RM6100 Rf4 gene. One hundred five possessing either or both genes test crossed with CMS line COMS 23A. In 67 hybrids evaluated, mean pollen fertility ranged from 97.3% (CB174R/Azucena 177-4-9) 13.7% 13-2-4). frequency restorers high (49.25%) followed by partial (29.85%) maintainers (20.90%). selection efficiency DRRM- RF3-10 75.75% 54.54% respectively. Segregation among individual within a family witnessed molecular phenotypic data. Based on marker information, it concluded advance 53.3% F5 generation isolate stable that can be exploited in future produce highly heterotic rice.